Oxidants and Antioxidants Research Group

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Research Objectives


  • To decipher the molecular pathways of redox-mediated circuits in cell fate signaling.
  • To study the involvement and or impact of cellular redox status on human physiology and pathological states using in vitro and in vivo model systems with the objective of identifying novel targets for potential biomarker and/or therapeutic development
  • To study the effect of oxidants and anti-oxidants (naturally occurring or synthetic) on disease models with the aim of developing experimental therapeutic strategies



Research Areas


  • Oxidative stress and antioxidants in health and disease
  • Cellular mechanisms of toxicity by abnormal proteins in neurodegenerative disease
  • The role of lipid peroxidation as a possible prognostic factor for the development of diabetes
  • Oxidative DNA damage in the nuclear and mitochondrial genomes, its molecular relationship to mutation cancer and ageing and its possible modulation by diet
  • Ageing in the nematode Caenorhabditis elegans
  • Superoxide-dependent survival pathways in tumor cells
  • Receptor and non-receptor Death Signaling
  • Regulation of cell death signaling in cancer cells
  • Molecular mechanisms of reactive species damage and antioxidant protection in human disease
  • Reactive oxygen Species and Cell fate decisions
  • Bcl-2 family and mitochondrial physiology
  • Redox status and cancer stem cells
  • Autophage: regulatory mechanisms and biological functions in health and diseases
  • Antioxidants in food and cosmetic emulsion systems
  • Isolating, purifying, testing in vivo, and examining the mechanism of action of antioxidants from regional goods and traditional Chinese medicines
  • Influence of dietary components and antioxidants, on human health and disease
  • Analytical techniques for measuring damage biomarkers and antioxidants in biological samples
  • Identification and validation of biomarkers for environmental exposure
  • Chemoprevention
  • Role of metals/lipids in neurodegeneration
  • Orofacial pain mechanisms
  • Applications and effects of engineered nanomaterials
  • Involvement of reactive oxygen/nitrogen species and oxidative stress in cell death signaling pathways (apoptotic and non-apoptotic cell death)
  • Bioenergetics of human skin fibroblasts
  • Sulfite-mediated toxicity
  • Mechanisms of action of synthetic and natural antioxidants against atherosclerotic vascular disease
  • Human mineral and trace element metabolism
  • Applications of lipidomics to the drug and biomarker development process
  • Amyloid precursor protein biology in neuronal ageing and degeneration




Programme Highlights


June 2009 to June 2012
PI: Prof Barry Halliwell
NMRC grant to study the correlation between oxidative damage and wound healing outcomes


Jan 2008 to Dec 2010
PI: Prof Shazib Pervaiz
BMRC grant to study the effect of Bcl-2 expression on mitochondrial physiology in tumor cells


2008-2011
PI: A/P Marie-Veronique Clement
NMRC grant to study the mechanism(s) involved in the down-regulation of the NA+/H+ exchanger 1 gene expression by peroxisome proliferator-activated receptor-gamma (PPARγ): a possible new strategy to selectively sensitize tumor cells to anticancer drug treatment


Jan 2009 to Dec 2011
PI: Prof Shazib Pervaiz
BMRC grant to study the involvement of reactive oxygen species and RHO GTPASE in statin-induced apoptosis of breast cancer cells


Jul 2009 to Jul 2012
PI: Prof Shazib Pervaiz
NMRC grant to study the transcriptional regulation of cFLIP in glucose deprivation-induced sensitization of Bcl-2 overexpressing cancer cells to death receptor mediated apoptosis


Aug 2007 to Jul 2010
PI: Dr Huang Dejian
SERC grant to study the Quantitative Detection of Reactive Oxygen Species in Biological Systems Using Quantum Dots as Ultrasensitive Tags



Research Achievements


Awards


Prof Barry Halliwell
Lifetime Achievement Award, Society for Free Radical Biology and Medicine (SFRBM)


A/Prof Shen Han Ming
Most cited paper in Free Radical Biology and Medicine


Prof Shazib Pervaiz
Received the award for "Best Paper Published in 2007" in the journal Cytometry A, at the Annual Meeting of the International Society for the Advancement of Cytomtery (ISAC), held in Budapest, Hungary


Patents


Prof Shazib Pervaiz
2008
Metal triangulo compound and methods of using the same.
Patent Application No: BRC/P/04351/04/SG
March 2009
A ligand for the peroxisome proliferation activator receptor-gamma.
Patent Application No: BRC/P/05281/00/US.


Key Representative Publications


  1. B Halliwell, JMC Gutteridge. Free Radicals in Biology and Medicine, 4th edition, Oxford University Press, UK (2007).
  2. Lee CY, Seet RC, Huang SH, Long LH, Halliwell B. Different patterns of oxidized lipid products in plasma and urine of dengue fever, stroke and Parkinsons disease patients. Cautions in the use of biomarkers of oxidative stress. Antioxid. Redox Signal. 11:407-420. (2009).
  3. Gruber J, Schaffer S, Halliwell B. The mitochondrial free radical theory of ageing - where do we stand? Front. Biosci. 13:6554-6579. (2008).
  4. Shenoy K, Wu Y, Pervaiz S. LY303511 enhances TRAIL sensitivity of SHEP-1 neuroblastoma cells via hydrogen peroxide-mediated mitogen-activated protein kinase activation and up-regulation of death receptors. Cancer Research. 69(5):1941-50, 2009.
  5. Kumar AP, Quake AL, Chang MK, Zhou T, Lim KS, Singh R, Hewitt RE, Salto-Tellez M, Pervaiz S, Clément MV. Repression of NHE1 expression by PPARgamma activation is a potential new approach for specific inhibition of the growth of tumor cells in vitro and in vivo. Cancer Research, 15;69(22):8636-44, 2009.
  6. Lee CY, Huang SH, Jenner AM, Halliwell B. Measurement of F2- isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample. Free Rad. Biol. Med. 44:1314-1322. (2008).
  7. Kumar AP, Chang MK, Fliegel L, Pervaiz S, Clement MV. Oxidative repression of NHE1 gene expression involves iron-mediated caspase activity. Cell Death Differ. 14(10): 1733-46, 2007
  8. Chen, Z, X. and Pervaiz, S. Bcl-2 Induces Pro-oxidant State by Engaging Mitochondrial Respiration in Tumor Cells. Cell Death Diff. 14(9): 1617-27, 2007.
  9. Chen, ZX and Pervaiz, S. Involvement of the cytochrome C oxidase subunits Va and Vb in the regulation of cancer cell metabolism by Bcl-2. Cell Death Diff. 2009
  10. Huang Q, Wu YT, Tan HL, Ong CN, Shen HM. A novel function of poly(ADP-ribose) polymerase-1 in modulation of autophagy and necrosis under oxidative stress. Cell Death Differ. 2009 Feb;16(2):264-77



Member(s) of programme


Programme Coordinator:
Prof Shazib Pervaiz


Members:
Prof Barry HalliwellAssoc Prof Thomas Walczyk
Prof Ong Choon NamAssoc Prof Shen Han Ming
Prof Sit Kim PingAssoc Prof Markus Wenk
Prof Frank WattDr Wong Boon Seng
Prof Roland StockerDr Huang Dejian
Assoc Rajesekhar BalasubramanianDr Ronan Kelly
Assoc Prof Tan Kwong Huat BennyDr Jan Gruber
Assoc Prof Ong Wei YiDr Andrew Jenner
Assoc Prof Marie-Veronique Clement Dr Lee Chung-Yung, Jetty



Contact Details


Professor Shazib Pervaiz
Department of Physiology
Yong Loo Lin School of Medicine
National University of Singapore
Blk MD9, 2 Medical Drive
Singapore 117597
Tel : 6516 6602
Fax : 6778 8161
Email : phssp@nus.edu.sg
Website : http://www.med.nus.edu.sg/research/progrsch/oxidants.shtml