Allergy Asthma and Immunology Research

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Research Interests

 

  1. Prevention and treatment of asthma and allergic diseases
  2. Immunopathogenesis of asthma and allergic diseases
  3. Microbe-human interactions on the development of allergy
  4. Epidemiology of asthma and allergic diseases
  5. Study of immuno-regulatory approaches and their applications on the control of allergic Diseases

 

Research Areas


A) Mechanistic Studies

  1. Allergen-cell interaction
  2. Mechanism of DNA vaccination
  3. Mechanism of lactic acid bacteria based vaccination

 

B) Translational Research

  1. Vaccine and Therapy
    • Recombinant mite allergen-based immunotherapy
    • DNA vaccine-based immunotherapy
    • Oral vaccine based immunotherapy
    • Lactic acid bacteria based immunotherapy
  2. Diagnostic
    • Production of recombinant mite allergens
    • Development of diagnostic kits

 

C) Epidemiological/ Multidisciplinary Clinical Studies

  1. To evaluate the role of probiotics in the prevention of allergic diseases
  2. To study the immune responses to allergens in early childhood in relation to environmental factors
  3. To study the clinical and molecular aspects of cross-reactivity between dust mite allergen and shellfish allergy
  4. To comparatively evaluate the composition of stool microbiome and their metagenome for infant with and without eczema
  5. To study the relationship of upper airway respiratory viral infection in young children with persistent rhinitis
  6. To evaluate a unique IgE-mediated allergy/anaphylaxis to galacto-oligosaccharede, a commercial prebiotics

 

 

Programme Highlights


Therapeutic and prevention modality with respect to studies on:

 

  • Development of DNA Vaccine for Asthma and Allergic diseases
  • Development of lactobacilli - based oral vaccine for protective immune responses against allergy
  • Recombinant mite allergens - based immunotherapeutic vaccine for allergy
  • Study of immunoregulatory proteins and their application in treatment of allergic diseases
  • Evaluation of food allergens such as shellfish and bird's nest

 


Influence of environmental factors in the development of childhood allergies:

  • A clinical trial on the effects of probiotics on atopic disease in babies at risk for atopy
  • Molecular studies of stool microbiota and its influence on eczema. 
  • Evaluation of respiratory viral infection and its influence on persistent rhinitis
  • Studies on the prevalence, IgE epitope and primary sensitizer of allergy to galacto-oligosaccharide

 

 

Collaborative Research Projects

  • University of Illinois (US), King Abdullah University of Science and Technology (Saudi Arabia): Comparative study of gut microbiota and metagenome with the development of allergic diseases.
  • Regional multi-centre epidemiological studies of mite allergy in patients with asthma/rhinitis.

 

 

 

Research Achievements

  1. Recent Major research achievements
    1. Two important breakthroughs in the field of allergy:
      1. Determined the 3D structure for a major dust mite allergen Blo t 5 that sensitized about 1 billion people worldwide.
      2. Generated the Blo t 5 allergen-specific T cell receptor transgenic mice

     

     

  2. Selected Publications since 2008
  3.  

    1. Chiang WC, Huang CH, Llanora GV, Gerez I, Goh SH, Shek LP, Nauta AJ, Van Doorn WA, Bindels J, Ulfman LH, Knipping K, Delsing DJ, Knol EF, Lee BW. Anaphylaxis to cow's milk formula containing short-chain galacto-oligosaccharide. J Allergy Clin Immunol. 2012. 130(6):1361-7.
    2. Connett GJ, Gerez I, Cabrera-Morales EA, Yuenyongviwat A, Ngamphaiboon J, Chatchatee P, Sangsupawanich P, Soh SE, Yap GC, Shek LP, Lee BW: A population-based study of fish allergy in the Philippines, Singapore and Thailand. Int Arch Allergy Immunol. 2012. 159(4):384-90.
    3. Su CF, Kuo IC, Chen PW, Huang CH, Seow SV, Chua KY, Yu SM. Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture. Transgenic Res. 2012. 21(1):177-92.
    4. Quah PL, Kuo IC, Huang CH, Shek LP, Lee BW, Chua KY: Early onset wheeze associated with enhanced combined IL-1beta, IL-6, and IL-12/IL-23p40 in LPS-stimulated cord blood mononuclear cells. Clin Exp Allergy. 2011. 41(7):970-8.
    5. Yap GC, Chee KK, Hong PY, Lay C, Satria CD, Sumadiono, Soenarto Y, Haksari EL, Aw M, Shek LP, Chua KY et al: Evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy. BMC Microbiol. 2011. 11:193.
    6. Huang CH, Loo EX, Kuo IC, Soh GH, Goh DL, Lee BW, Chua KY. Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35. J Immunol. 2011. 187(1):462-71.
    7. Hong, P.-Y., B.W. Lee, M. Aw, L.P.C. Shek, G.C. Yap, K.Y. Chua and W.-T. Liu (2010) Comparative analysis of fecal microbiota in infants with and without eczema. PLoS One. 5(4): e9964.
    8. Shek LP, Cabrera-Morales EA, Soh SE, Gerez I, Ng PZ, Yi FC, Ma S, Lee BW. A population-based questionnaire survey on the prevalence of peanut, tree nut, and shellfish allergy in 2 Asian populations. J Allergy Clin Immunol. 2010. 126(2):324-31.
    9. Shek LP, Chong AR, Soh SE, Cheong N, Teo AS, Yi FC, Giam YC, Chua KY, Van Bever HP. Specific profiles of house dust mite sensitization in children with asthma and in children with eczema. Pediatr Allergy Immunol. 2010. 21(4 Pt 2):e718-22.
    10. Liedén A, Ekelund E, Kuo IC, Kockum I, Huang CH, Mallbris L, Lee SP, Seng LK, Chin GY, Wahlgren CF, Palmer CN, Björkstén B, Ståhle M, Nordenskjöld M, Bradley M, Chua KY, D'Amato M. Cornulin, a marker of late epidermal differentiation, is down-regulated in eczema. Allergy. 2009. 64(2):304-11.
    11. Ding Y, Seow SV, Huang CH, Liew LM, Lim YC, Kuo IC, and Chua KY, Coadministration of the fungal immunomodulatory protein FIP-Fve and a tumour-associated antigen enhanced antitumour immunity. Immunology. 2009. 128(1 Suppl):e881-94.
    12. Soh SE, Aw M, Gerez I, Chong YS, Rauff M, Ng YP, Wong HB, Pai N, Lee BW, Shek LP. Probiotic supplementation in the first 6 months of life in at risk Asian infants--effects on eczema and atopic sensitization at the age of 1 year. Clin Exp Allergy. 2009. 39(4):571-8.
    13. Yi FC, Chen JY, Chee KK, Chua KY, Lee BW. Dust mite infestation of flour samples. Allergy. 2009. 64(12):1788-9.
    14. Chua KY, Kuo IC, Huang CH. DNA vaccines for the prevention and treatment of allergy. Curr Opin Allergy Clin Immunol. 2009. 9(1):50-4.
    15. Xu H, Liew LN, Kuo IC, Huang CH, Goh DL, Chua KY. The modulatory effects of lipopolysaccharide-stimulated B cells on differential T-cell polarization. Immunology. 2008. 125(2):218-28.
    16. Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH. Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen. Structure. 2008. 16(1):125-36.
    17. Lim DL, Neo KH, Yi FC, Chua KY, Goh DL, Shek LP, Giam YC, Van Bever HP, Lee BW. Parvalbumin--the major tropical fish allergen. Pediatr Allergy Immunol. 2008. 19(5):399-407.

     


    Member(s) of Programme

     

        A/Prof. Lynette Shek Pei Chi
        Adj Prof. Lee Bee Wah
        Prof. Van Bever, Hugo P S
        Dr. Huang Chiung Hui
        Dr. Kuo I-Chun
        Mr. Haris
        Mr. Antony Hardjojo
         
        Ms. Chua Peng Yian
        Ms. Yap Gaik Chin
        Ms. Wen Hong Mei
        Ms. Chua Lee Hui
        Ms. Chong Ming Hua
        Ms. Lee Li Yuan, Gabriella Nadine
        Ms. Pang Yu Hui, Delia
         

     

     

    Contact Details


    A/Prof. Lynette Shek Pei Chi

    Department of Paediatrics

    Yong Loo Ling School of Medicine

    #03-16, MD11, Clinical Research Centre

    10 Medical Drive

    Singapore 117597

    Tel: 6772 3353

    e-mail: allergy_kids@nuhs.edu.sg

     

    Adj Prof. Lee Bee Wah

    Department of Paediatrics

    Yong Loo Ling School of Medicine

    #03-19, MD11, Clinical Research Centre

    10 Medical Drive

    Singapore 117597

    e-mail: paeleebw@nus.edu.sg

    Prof. Van Bever, Hugo P S

    Department of Pardiatrics

    Yong Loo Ling School of Medicine

    NUHS Tower Block Level 12

    1E Kent Ridge Road

    Singapore 119228

    Tel: 6772 4417

    e-mail: hugo_van_bever@nuhs.edu.sg

     

    Dr. Huang Chiung-Hui

    Department of Paediatrics

    Yong Loo Ling School of Medicine

    #03-15, MD11, Clinical Research Centre

    10 Medical Drive

    Singapore 117597

    Tel: 6516 4952

    e-mail: paehch @nus.edu.sg

    Dr. Kuo I-Chun

    Department of Paediatrics

    Yong Loo Ling School of Medicine

    #03-14, MD11, Clinical Research Centre

    10 Medical Drive

    Singapore 117597

    Tel: 6516 4952

    e-mail: paekic@nus.edu.sg